Hey! I am trying to do colour deconvolution on some images, so I copied my macro into the ImageJ Macro pure code:
run(“Stack to RGB”);
run(“Subtract Background…”, “rolling=70 light”);
run(“Colour Deconvolution”, “vectors=[User values] hide [r1]=29.831186 [g1]=66.10667 [b1]=97.59406 [r2]=40.33258 [g2]=37.12847 [b2]=13.552562 [r3]=0.44360253 [g3]=2.2902856 [b3]=1.5317869”);
However, I get the following warning:
WARN ImageJ Macro 4:2231 ImageJ Macro Error: Unrecognized command: “Colour Deconvolution”
WARN ImageJ Macro 4:2231 Problem occured in rowTMA 101 B7H4 IHC-Scene-06-ScanRegion5.czi_1 : The specified macro has thrown an error while execution. Make sure that the used plugins are available in the selected IJ1 plugin folder! See KNIME Log for details! Missing cell has been inserted
Is it because colour deconvolution can’t run headless? Or am i doing something wrong? Is there a way around it? Can it be added as an imageJ2 pluggin?