I am very much interested in the dissection of cells from pictures with nuclei stain (hoechst) and cytoplasm stain (phalloidin). I tried in Knime several methods to dissect the cytoplasm (voronoi using nuclei as seeds or watershed with the same seeds) however the resulting segmentations were not verry good.
However previsously I was working with propagation method in CellProfiler and this method provided solid results, here is a link to the publication:
unfortunately this method is not available in KNIME Image Processing, ...yet. I have put it on our list for algorithmswe want to integrate. thanks for the link!
Anyway, you have two options:
* If you want you can still use this method and do your measurements in CellProfiler and propagate the results back to KNIME. We have just released a CellProfiler integration for that (see: https://tech.knime.org/community/cellprofiler)
* The Voronoi-Segmentation is the closest we have to the method which is described in the attached paper. Maybe tweaking the parameters helps there (e.g. setting a threshold or preprocessing the images with some filter).
I can't tell you a date, yet. But it's of course an algorithm we want to make available in KNIME. Do you know about any ImageJ plugin implementing this functionality? Then an integration would be pretty simple and I could do it immediately.