I am trying to align images from a time laps recording to compensate for very small shifts in X,Y dimensions. There is an example worflow available on the KNIME hub that should work in principle
However, this example is for a multi-channel coloured image. My time laps images are grey scale. I tried several options to merge my images to obtain stacks of either a reference images (the first frame) and a subsequent images or by creating a stack of all images and then aligning among the time channel.
In both cases I received error messages, e.g.
- Problem occured in rowRow0 : Wrong number of valid shift dimensions ‘2’ or dimensions do not exist in Image! Skipping Image Img1 01 01_034–t01.tif Missing cell has been inserted
- Problem occured in rowImage : Wrong number of valid shift dimensions ‘2’ or dimensions do not exist in Image! Skipping Image Img1 01 01_034–t20.tif Missing cell has been inserted
The appropriate workflow I tried to set up can be downloaded here (190 MB, the Link-password is “knimeforum”):
I assume that the way how I merged the images to obtain a multichannel object is not correct. Do I really need channels with different colours? Hope there is a simple answer to solve this problem. Thanks a lot in advance and kind regards.