Is there a way to apply a pre- defined layout (in my case, a simple 96 well microplate) to images of plates to create objects/ regions of interest (the single wells). The image consists of a photo of the plate (each image depicts full cell culture plate) with all the 8 x 12 wells. I would like to superimpose the mask/ layout of 8 x 12 circles which are my regions of interest, inside which I want to measure pixel intensities.