Dear all,
I'm trying to get openMS working under KNIME 2.11.3 (the computer runs under windows 7, 64 bits). I'm particularly interested on performing alignment of LC-MS runs. I downloaded the pipeline referred in Aiche et al. Proteomics 2015 (wrokflow 2, label-free quantification of metabolites). I also donwloaded the corresponding input files, corrected the node "input files" (so that now KNIME has the right paths to the LC-MS data), and started. However, I get problems with MapAlignerPoseClustering and FeatureLinkerUnlabeledQT.
ERROR FeatureFinderMetabo Loading model settings failed: String for key "FeatureFinderMetabo.1.force" not found.
ERROR MapAlignerPoseClustering Loading model settings failed: String for key "MapAlignerPoseClustering.1.force" not found.
WARN FeatureLinkerUnlabeledQT Invalid MIMEtype at port number 0 : has extension: [application/x-mzml]; expected on of:[[featurexml, consensusxml]]
ERROR LoadWorkflowRunnable Errors during load: Status: Error: Example2_Metabolite_ID_Workflow 3 loaded with errors
ERROR LoadWorkflowRunnable Status: Error: Example2_Metabolite_ID_Workflow 3
ERROR LoadWorkflowRunnable Status: Error: FeatureFinderMetabo 3:106
ERROR LoadWorkflowRunnable Status: Error: Loading model settings failed: String for key "FeatureFinderMetabo.1.force" not found.
ERROR LoadWorkflowRunnable Status: Error: MapAlignerPoseClustering 3:29
ERROR LoadWorkflowRunnable Status: Error: Loading model settings failed: String for key "MapAlignerPoseClustering.1.force" not found.
To debug, I truncated the pipeline, deleting all the nodes after FeatureLinkerUnlabeledQT and placing a generic Output Folder node after it:
InputFiles->ZipLoopStart->FeatureFinderMetabo->ZipLoopEnd->MapAlignerPoseClustering->FeatureLinkerUnlabeledQT->OutputFolder
If I try to run it again, FeatureFinderMetabo works and the pipeline stucks after MapAlignerPoseClustering; all the nodes are green except for FeatureLinkerUnlabeledQT and OutputFiles, which are marked red. The log message is:
WARN FeatureLinkerUnlabeledQT Invalid MIMEtype at port number 0 : has extension: [application/x-mzml]; expected on of:[[featurexml, consensusxml]]
WARN FeatureLinkerUnlabeledQT Invalid MIMEtype at port number 0 : has extension: [application/x-mzml]; expected on of:[[featurexml, consensusxml]]
WARN FeatureLinkerUnlabeledQT Invalid MIMEtype at port number 0 : has extension: [application/x-mzml]; expected on of:[[featurexml, consensusxml]]
I don't know if the following small trouble I got with standalone OpenMS 1.12 and 2.0 (win 7, 64 bits) is somehow related to this or if this can help with the troubleshooting. In standalone OpenMS I could only get MapAlignerPoseClustering and FeatureLinkerUnlabeledQT working properly in similar pipelines if I placed a Collector node immediately before MapAlignerPoseClustering. I don' t find such a Collector node under KNIME.
If you need more details or files, please let me know.
Best wishes
Ana