Hello all,
I am trying to test the Assaygeneratometabo, however I keep getting an Error. Can someone please help me where the problem is?
With best regards,
Adrian Brun
ERROR AssayGeneratorMetabo 3:375 Input /private/var/folders/hg/f843c26s3qj05rrywx_1pps00000gn/T/20221124_153834_Adrians-MacBook-Pro.local_8723_8.ms --project /private/var/folders/hg/f843c26s3qj05rrywx_1pps00000gn/T/20221124_153834_Adrians-MacBook-Pro.local_8723_7/sirius_out sirius --db=all --tree-timeout=0 --candidates=5 --profile=qtof --ppm-max=10.0 --compound-timeout=100 --ppm-max-ms2=10.0 passatutto , Executable is: /Applications/SiriusContents/MacOS/sirius, COMMAND: /Applications/SiriusContents/MacOS/sirius --processors=1 --input /private/var/folders/hg/f843c26s3qj05rrywx_1pps00000gn/T/20221124_153834_Adrians-MacBook-Pro.local_8723_8.ms --project /private/var/folders/hg/f843c26s3qj05rrywx_1pps00000gn/T/20221124_153834_Adrians-MacBook-Pro.local_8723_7/sirius_out sirius --db=all --tree-timeout=0 --candidates=5 --profile=qtof --ppm-max=10.0 --compound-timeout=100 --ppm-max-ms2=10.0 passatutto, Executing: /Applications/SiriusContents/MacOS/sirius, Sirius Workspace was successfully copied to /Users/adrianbrun/Desktop/Diametalyzer, Warning: Original paths of the mzML files do not correspond to the featureXML files. Please check and provide the corresponding files., Input MzML: /private/var/folders/hg/f843c26s3qj05rrywx_1pps00000gn/T/knime_DMA_test84307/fs-ZipLoopStart-84308/000/000/38_4_HighResPrecursorMassCorrector_0/PestMix5_1ngSolventDDA20-50.mzML, Input FeatureXML: /private/var/folders/hg/f843c26s3qj05rrywx_1pps00000gn/T/knime_DMA_test84307/fs-ZipLoopStart-84308/000/000/37_4_AccurateMassSearch_1/PestMix5_1ngSolventDDA20-50.featureXML, Original paths: , /Users/adrianbrun/Documents/Diametalyzer/DDA/PestMix5_1ngSolventDDA20-50.mzML, Correction to the highest intensity peak failed 782 times because of missing peaks in the MS1. No changes were applied in these cases., No MS1 spectrum for this precursor. Occurred 0 times., 0 spectra were skipped due to precursor charge below -1 and above +1., Mono charge assumed and set to charge 1 with respect to current polarity 80 times., 0 features were skipped due to feature charge below -1 and above +1., Number of features to be processed: 52, Running SIRIUS with the following command line parameters: , --processors=1 --input /private/var/folders/hg/f843c26s3qj05rrywx_1pps00000gn/T/20221124_153854_Adrians-MacBook-Pro.local_8723_10.ms --project /private/var/folders/hg/f843c26s3qj05rrywx_1pps00000gn/T/20221124_153854_Adrians-MacBook-Pro.local_8723_9/sirius_out sirius --db=all --tree-timeout=0 --candidates=5 --profile=qtof --ppm-max=10.0 --compound-timeout=100 --ppm-max-ms2=10.0 passatutto , Executable is: /Applications/SiriusContents/MacOS/sirius, COMMAND: /Applications/SiriusContents/MacOS/sirius --processors=1 --input /private/var/folders/hg/f843c26s3qj05rrywx_1pps00000gn/T/20221124_153854_Adrians-MacBook-Pro.local_8723_10.ms --project /private/var/folders/hg/f843c26s3qj05rrywx_1pps00000gn/T/20221124_153854_Adrians-MacBook-Pro.local_8723_9/sirius_out sirius --db=all --tree-timeout=0 --candidates=5 --profile=qtof --ppm-max=10.0 --compound-timeout=100 --ppm-max-ms2=10.0 passatutto, Executing: /Applications/SiriusContents/MacOS/sirius, Sirius Workspace was successfully copied to /Users/adrianbrun/Desktop/Diametalyzer, Warning: Original paths of the mzML files do not correspond to the featureXML files. Please check and provide the corresponding files., Input MzML: /private/var/folders/hg/f843c26s3qj05rrywx_1pps00000gn/T/knime_DMA_test84307/fs-ZipLoopStart-84308/000/000/46_5_HighResPrecursorMassCorrector_0/PestMix6_1ngSolventDDA20-50.mzML, Input FeatureXML: /private/var/folders/hg/f843c26s3qj05rrywx_1pps00000gn/T/knime_DMA_test84307/fs-ZipLoopStart-84308/000/000/45_5_AccurateMassSearch_1/PestMix6_1ngSolventDDA20-50.featureXML, Original paths: , /Users/adrianbrun/Documents/Diametalyzer/DDA/PestMix6_1ngSolventDDA20-50.mzML, Warning: no MS1 spectrum for this precursor, Correction to the highest intensity peak failed 760 times because of missing peaks in the MS1. No changes were applied in these cases., No MS1 spectrum for this precursor. Occurred 0 times., <Warning: no MS1 spectrum for this precursor> occurred 6 times, 0 spectra were skipped due to precursor charge below -1 and above +1., Mono charge assumed and set to charge 1 with respect to current polarity 20 times., 0 features were skipped due to feature charge below -1 and above +1., Number of features to be processed: 32, Running SIRIUS with the following command line parameters: , --processors=1 --input /private/var/folders/hg/f843c26s3qj05rrywx_1pps00000gn/T/20221124_153912_Adrians-MacBook-Pro.local_8723_12.ms --project /private/var/folders/hg/f843c26s3qj05rrywx_1pps00000gn/T/20221124_153912_Adrians-MacBook-Pro.local_8723_11/sirius_out sirius --db=all --tree-timeout=0 --candidates=5 --profile=qtof --ppm-max=10.0 --compound-timeout=100 --ppm-max-ms2=10.0 passatutto , Executable is: /Applications/SiriusContents/MacOS/sirius, COMMAND: /Applications/SiriusContents/MacOS/sirius --processors=1 --input /private/var/folders/hg/f843c26s3qj05rrywx_1pps00000gn/T/20221124_153912_Adrians-MacBook-Pro.local_8723_12.ms --project /private/var/folders/hg/f843c26s3qj05rrywx_1pps00000gn/T/20221124_153912_Adrians-MacBook-Pro.local_8723_11/sirius_out sirius --db=all --tree-timeout=0 --candidates=5 --profile=qtof --ppm-max=10.0 --compound-timeout=100 --ppm-max-ms2=10.0 passatutto, Executing: /Applications/SiriusContents/MacOS/sirius, Sirius Workspace was successfully copied to /Users/adrianbrun/Desktop/Diametalyzer, Warning: Original paths of the mzML files do not correspond to the featureXML files. Please check and provide the corresponding files., Input MzML: /private/var/folders/hg/f843c26s3qj05rrywx_1pps00000gn/T/knime_DMA_test84307/fs-ZipLoopStart-84308/000/000/54_6_HighResPrecursorMassCorrector_0/PestMix7_1ngSolventDDA20-50.mzML, Input FeatureXML: /private/var/folders/hg/f843c26s3qj05rrywx_1pps00000gn/T/knime_DMA_test84307/fs-ZipLoopStart-84308/000/000/53_6_AccurateMassSearch_1/PestMix7_1ngSolventDDA20-50.featureXML, Original paths: , /Users/adrianbrun/Documents/Diametalyzer/DDA/PestMix7_1ngSolventDDA20-50.mzML, Warning: no MS1 spectrum for this precursor, Correction to the highest intensity peak failed 799 times because of missing peaks in the MS1. No changes were applied in these cases., No MS1 spectrum for this precursor. Occurred 0 times., <Warning: no MS1 spectrum for this precursor> occurred 2 times, 0 spectra were skipped due to precursor charge below -1 and above +1., Mono charge assumed and set to charge 1 with respect to current polarity 44 times., 0 features were skipped due to feature charge below -1 and above +1., Number of features to be processed: 42, Running SIRIUS with the following command line parameters: , --processors=1 --input /private/var/folders/hg/f843c26s3qj05rrywx_1pps00000gn/T/20221124_153927_Adrians-MacBook-Pro.local_8723_14.ms --project /private/var/folders/hg/f843c26s3qj05rrywx_1pps00000gn/T/20221124_153927_Adrians-MacBook-Pro.local_8723_13/sirius_out sirius --db=all --tree-timeout=0 --candidates=5 --profile=qtof --ppm-max=10.0 --compound-timeout=100 --ppm-max-ms2=10.0 passatutto , Executable is: /Applications/SiriusContents/MacOS/sirius, COMMAND: /Applications/SiriusContents/MacOS/sirius --processors=1 --input /private/var/folders/hg/f843c26s3qj05rrywx_1pps00000gn/T/20221124_153927_Adrians-MacBook-Pro.local_8723_14.ms --project /private/var/folders/hg/f843c26s3qj05rrywx_1pps00000gn/T/20221124_153927_Adrians-MacBook-Pro.local_8723_13/sirius_out sirius --db=all --tree-timeout=0 --candidates=5 --profile=qtof --ppm-max=10.0 --compound-timeout=100 --ppm-max-ms2=10.0 passatutto, Executing: /Applications/SiriusContents/MacOS/sirius, Sirius Workspace was successfully copied to /Users/adrianbrun/Desktop/Diametalyzer, Warning: Original paths of the mzML files do not correspond to the featureXML files. Please check and provide the corresponding files., Input MzML: /private/var/folders/hg/f843c26s3qj05rrywx_1pps00000gn/T/knime_DMA_test84307/fs-ZipLoopStart-84308/000/000/62_7_HighResPrecursorMassCorrector_0/PestMix8_1ngSolventDDA20-50.mzML, Input FeatureXML: /private/var/folders/hg/f843c26s3qj05rrywx_1pps00000gn/T/knime_DMA_test84307/fs-ZipLoopStart-84308/000/000/61_7_AccurateMassSearch_1/PestMix8_1ngSolventDDA20-50.featureXML, Original paths: , /Users/adrianbrun/Documents/Diametalyzer/DDA/PestMix8_1ngSolventDDA20-50.mzML, Warning: no MS1 spectrum for this precursor, Correction to the highest intensity peak failed 720 times because of missing peaks in the MS1. No changes were applied in these cases., No MS1 spectrum for this precursor. Occurred 0 times., <Warning: no MS1 spectrum for this precursor> occurred 8 times, 0 spectra were skipped due to precursor charge below -1 and above +1., Mono charge assumed and set to charge 1 with respect to current polarity 40 times., 0 features were skipped due to feature charge below -1 and above +1., Number of features to be processed: 31, Running SIRIUS with the following command line parameters: , --processors=1 --input /private/var/folders/hg/f843c26s3qj05rrywx_1pps00000gn/T/20221124_153945_Adrians-MacBook-Pro.local_8723_16.ms --project /private/var/folders/hg/f843c26s3qj05rrywx_1pps00000gn/T/20221124_153945_Adrians-MacBook-Pro.local_8723_15/sirius_out sirius --db=all --tree-timeout=0 --candidates=5 --profile=qtof --ppm-max=10.0 --compound-timeout=100 --ppm-max-ms2=10.0 passatutto , Executable is: /Applications/SiriusContents/MacOS/sirius, COMMAND: /Applications/SiriusContents/MacOS/sirius --processors=1 --input /private/var/folders/hg/f843c26s3qj05rrywx_1pps00000gn/T/20221124_153945_Adrians-MacBook-Pro.local_8723_16.ms --project /private/var/folders/hg/f843c26s3qj05rrywx_1pps00000gn/T/20221124_153945_Adrians-MacBook-Pro.local_8723_15/sirius_out sirius --db=all --tree-timeout=0 --candidates=5 --profile=qtof --ppm-max=10.0 --compound-timeout=100 --ppm-max-ms2=10.0 passatutto, Executing: /Applications/SiriusContents/MacOS/sirius, Sirius Workspace was successfully copied to /Users/adrianbrun/Desktop/Diametalyzer]
ERROR AssayGeneratorMetabo 3:375 Failing process stderr:
ERROR AssayGeneratorMetabo 3:375 Return code: 139
ERROR AssayGeneratorMetabo 3:375 Execute failed: Failed to execute node AssayGeneratorMetabo