This tutorial workflow shows a simple larva segmentation and tracking. Of course parameters and methods have to be adjusted for your problem, but this is a good starting point.
I tried this workflow. But there is a small issue. The Image Reader node can’t read proper information from the example tif image. The “Time” channel is reconized as “Unknown”. This cause the folloing Projector node won’t get the right channel which is “Time”. I don’t know this is a problem with the Image Reader node, or just the setup of my OS.
Thank you for the feedback, it is indeed a problem with the image reader, I will investigate further.
You can fix this issue with a Set Image Metadata node. See the following workflow for an example:
https://hub.knime.com/gab1one/space/02_Larva_Tracking-fixed