The workflow detects the number and frequency of spontaneous tail coilings displayed in zebrafish embryos through image analysis of videos in AVI format. The ffmpeg tool is required to split AVI files into image frames. Spontaneous tail coilings in zebrafish embryos were recorded with a video camera (Olympus DP21) mounted in an Olympus SZX7 stereomicroscope (0.8x magnification, camera with a minimal resolution of 120 frames per minute or more) using a black background and dark field transmitted light at the base. The black background is important in order to detect each egg on the images.
This is a companion discussion topic for the original entry at https://kni.me/w/3B1YHS6G4Uz5TWwk