Unable to determine OMSSA version; Make sure the OMSSA executable given in '-omssa_executable' is correct!

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1

I am following a tutorial from openms which involves a workflow using OMSSA in KNIME. Upon inputting my own work files and Uniprot database, I get the following error. It seems that KNIME is unable to find the executable file. I suspected it might be caused by the space in the KNIME application name (originally /Applications/KNIME 4.1.0.app/…). However, after changing to “/Applications/KNIME-4.1.0.app/…”, the problem still persists.

ERROR OMSSAAdapter 0:9 Failing process stdout: [Warning: unable to determine the version of OMSSA - the process returned an error. Call string was: ‘/Applications/KNIME-4.1.0.app/Contents/Eclipse/plugins/de.openms.macosx.x86_64_2.6.0.202004161737/payload/bin/OMSSA/omssacl -version’. Make sure that the OMSSA executable given in ‘-omssa_executable’ is correct!, OMSSAAdapter took 0.01 s (wall), 0.01 s (CPU), 0.00 s (system), 0.01 s (user).]
ERROR OMSSAAdapter 0:9 Failing process stderr:
ERROR OMSSAAdapter 0:9 Return code: 6
ERROR OMSSAAdapter 0:9 Execute failed: Failed to execute node OMSSAAdapter

Anyone encounter the same issue and knows the solution?

2

Hi!

Did you check manually if the executable is at the place it is trying to look for?
Call string was: ‘/Applications/KNIME-4.1.0.app/Contents/Eclipse/plugins/de.openms.macosx.x86_64_2.6.0.202004161737/payload/bin/OMSSA/omssacl

If it is there, can you try to open a command line there and execute it to see if it is executable?

3

Hi jpfeuffer,

Thanks for the reply. The executable is at the location mentioned however when I run it I get an error:

zsh: bad CPU type in executable: ./omssacl

4

Actually I can confirm on macOS Catalina. Unfortunately Apple dropped 32bit support
since Catalina. We will try to rebuild OMSSA but since it is to my knowledge not really maintained anymore, I cannot promise anything.
Until then (and also for future-proof pipelines) I would suggest to move to a newer search engine like Comet or MSGFPlus.
Certainly, we have to update the tutorials on our side.
You can follow the progress on this issue here: https://github.com/OpenMS/OpenMS/issues/4281